Influence of enzyme concentration on the Michaelis-Menten constant: A new theory for izolated cases

This study proposes a novel theory that challenges the traditional understanding of the Michaelis-Menten constant (Km) as a fixed value for a given enzyme-substrate pair. We explore the possibility that Km can be modulated by enzyme concentration. By analyzing the relationship between initial reaction velocity and substrate concentration at different enzyme concentrations, we observed a change in the apparent affinity constant (designated as Km1 and Km2). This observation contradicts established theory, leading us to propose a new model to explain this variation.