AHA 20CDA35310305 data

An intestine-specific <i>Scd1 </i>knockout model was developed by crossing <i>Scd1</i>^{<em>fl/fl</em>}<i> </i>mice with mice expressing Cre recombinase under the control of the Villin promoter to study the specific role of intestinal SCD1 in intestinal and whole-body lipid metabolism. The intestinal, hepatic and plasma lipid content and composition of these mice were evaluated by GC-MS analysis under chow fed and sucrose refed conditions. The role of intestinal SCD1 in the regulation of energy balance was also evaluated under chow fed and high-fat conditions. Bile acid content, composition, and signaling was analyzed. Additionally, metabolic phenotyping including body composition, indirect calorimetry and glucose tolerance analyses were conducted.