A Rapid Testing Kit for 6PPD Screening in Rubber Products

N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) has received increasing attention due to its ubiquitous environmental occurrence and extreme aquatic toxicity of its oxidation product 6PPD-quinone. Given 6PPD’s application as an antioxidant in a wide array of rubber products, cost-effective detection of 6PPD is important for product and waste management. We herein developed a rapid testing kit for the detection (N-1,3-dimethyl butyl-N’-phenyl quinone diimine (6QDI+H+), rather than its neutral or radical counterparts, appeared to be the red-colored product with λmax = 490 nm. We further developed a “green” and user-friendly 6PPD rapid testing kit, which was further applied to a wide array of 50 rubber products. 6PPD was detected in 22 out of 50 samples, with a strong agreement (R2 = 0.896) observed between the results of the rapid testing kit and LC-MS. In summary, this study introduces a 6PPD rapid testing kit for users without requiring expertise in chemistry.