Single-nucleus RNA sequencing of human fetal leptomeninges

To investigate the development of human leptomeninges and their co-development with the brain. Nuclei were extracted from eight human fetal leptomeninges obtained from aborted embryos, ranging from PCW8 to PCW21 (post-conceptional weeks). The entire arachnoid mater, pia mater, associated blood vessels, and immune cells were included for single-nucleus RNA sequencing (snRNA-seq). The leptomeninges intended for single-nucleus RNA sequencing were meticulously dissected from the forebrain of the cerebrum. The samples included the entire arachnoid mater, pia mater, associated blood vessels, and resident immune cells. After careful peeling, the leptomeninges were immersed in ice-cold collection buffer, washed with PBS, and then subjected to nuclear isolation.