Utilization of response surface methodology to optimize the process of extraction of Astragalus Polysaccharide and its impact on hyperglycemia and intestinal microflora in diabetic rats

Rat fecal samples were collected and sent to Beijing Tiangen Biochemical Technology Co., Ltd. for the construction of a high-throughput sequencing library, and paired-end sequencing was performed on the library based on the Illumina sequencing platform. The sample DNA was extracted and detected, and then the PCR products amplified by polymerase chain reaction (PCR) were quantified, characterized, mixed and purified. The sequencing library was constructed using the TIANSeq rapid DNA library construction kit, and then Qubit quantification and Agient2100 library detection were performed. After passing the detection, PE250bp sequencing was performed on the Illumina platform to obtain 250bp paired-end sequencing reads. Further analysis methods such as Alpha diversity analysis (including Chaol index, Shannon index, Ace index, and Simpson index), Beta diversity analysis, and taxonomic analysis (Venn diagram) were used to obtain the relevant flora information.