RNA-seq analysis of the DTA-injured retina

The goal of this study is to identify genes upregulated in the DTA-injured retina. Methods: Retinal mRNA profiles of control (DTAwt) and DTA (DTAmut) mice at day 1 and 5 post-injry were generated by deep-sequencing with Illumina sequencer. Results: RNA-seq tags were mapped to the mouse mm9 reference genome assembly using Tophat2 ver. 2.0.8b, with FPKM values caluculated with Cufflinks ver. 2.1.1 with the default option.Comparison of the genes upregulated in the DTA-injured retina at day 1 and 5 revealed commonly upregulated genes as well as the genes specific to day 1 and 5. Conclusions: Our study provides the detailed analysis of retinal transcriptomes in the DTA-injured retina. Overall design: Retinal mRNA profiles of retina harvested from 8-week-old control (DTAwt) or DTA (DTAmut) mice at day 1 or 5 post-injury were generated by deep-sequencing with Illumina Sequencer.