Barley embryo proteome analysis

To provide a more detailed insight into molecular changes in seeds exposed to the accelerated ageing process, we excised embryos and compared the differences in the proteome composition. Seeds collected from four contrasting locations were exposed to accelerated ageing. Next, embryos were dissected and analyzed in five biological replicates [1-5]. Sample description:CAS [1-5] - location n. 5 - (Caslav) - high yield, above average temperature and high rainfall; a significant decrease in germination energy after the accelerated ageing test. UHO [1-5] - location n. 11 (Uhersky Ostroh) - low yield, high temperature and low rainfall; very low decrease in germination energy after the accelerated ageing test.CH [1-5] - location n. 2 (Chrastava) - average yield, low temperature, very high rainfall; a significant decrease in germination energy after the accelerated ageing test.K [1-5] - location n. 8 (Krasne Udoli) - average yield, low temperature, average rainfall; a significant decrease in germination energy after the accelerated ageing test.