Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance

To Study the role of mutant Braf V600E and ERK dependent phosphorylation of TFEB in regulation of autophagy and lysosome biogenesis in the context of melanoma growth and metastasis, we used whole geneome RNA-seq to study role TFEB mediated BRAFV600E autophagy regulation. Overall design: For subcutaneous xenografts, A375 cells stably expressing WT TFEB or TFEB mutants (5 x 10^6) were injected subcutaneously into the lower flank of 5-6 six-week-old female NOD/SCID mice. After a three-weeks of post-inoculation, mice were sacrificed, and tumors were dissected and processed for RNA isolation.