Transcriptomics and Metabolomics analysis of Kcnj16 knockout rats

We hypothesized that deletion of Kcnj16 decreased the blood pressure and kidney damage by altering the inflammatory and metabolic pathways of Dahl Salt Sensitive Rats All rats used were male. At the age of 11-12 weeks old, rats were placed in metabolic cages for 24-hour urine collection. Prior to euthanasia, rats were anesthetized with isoflurane for arterial blood collection as well as kidney flushing with PBS. The left kidneys were removed and snapped frozen for RNA-sequencing analysis. All RNA were extracted from the cortex of the left kidneys.