How the whole genome amplification affect soil bacterial communities. soil metagenome

Whole genome amplification (WGA) has been used to amplify DNA from low biomass samples for downstream omics analysis with the risks of introducing biases and skewing community profiles. In this study, two WGA methods, Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC), were evaluated with three contrasting soils and by amplicon sequencing of 16S rRNA genes. The performance of these two WGA methods was compared in terms of the reconstruction of bacterial communities.