GNPS - Comparative genomics metabolomics analysis of Streptomyces species

Various MeOH and PBS extracts from Streptomyces species analyzed by LC-MS/MS analyzed in both positive and negative ionisation using a Vanquish UHPLC System coupled to a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific, United States). Chromatographic separation was performed in mixed mode (allowing weak anion/cation exchange) on a Scherzo SM-C18 column. Inclusion list was used for the positive mode, and background ions were excluded.