Genomic Glucocorticoid Action in Embryonic Mouse Neural Stem Cells. Genomic Glucocorticoid Action in Embryonic Mouse Neural Stem Cells

The goal of this study was to measure genome-wide expression in primary mouse neural stem/progenitor cell (NSPC) cultures to determine if SOX2 ablation alters the transcriptomic response which occurs following glucocorticoid receptor activation by the synthetic glucocorticoid, Dexamethasone. Neurosphere cultures of SOX2 knock out (KO) NSPCs and control non-deleted wild-type (WT) NSPCs (C57BL/6) derived from the fetal telencephalon were established at postnatal day zero (P0). Overall design: After the third passage, biologically distinct replicates of both sexes were treated in-vitro with 100nM Dexamethasone (Dex) for 4 hours and processed for microarray gene expression analysis (n=6 WT; n=7 SOX2 KO).