Effect of external standards mixture (ESM) on quality control.
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Inter-day experimental variation without and with ESM. Note: SM8: surgically removed, formalin-fixed, paraffin-embedded (FFPE) sample 8. ISM D (−12/−14) contains ACTB IS 10−12M/target gene IS 10−14M that corresponds to ACTB IS 600000/target gene IS 6000 molecules. ISM E (−12/−15) contains ACTB IS 600000/target gene IS 600 molecules. Cq: quantification cycle. ΔCq: quantification cycle difference of NT and IS: NT Cq - IS Cq. CV: coefficient of variation. NT: native template. IS: internal standard. ISM: internal standards mixture.
创建时间：
2014-02-21
