Reversal of age-related deficits in skeletal muscle during disuse and recovery in response to treatment with a secrotome product derived from partially differentiated human pluripotent stem cells

We report that the use of high throughput RNA sequencing in C2C12 myotubes induced myotobule hypertrophy and promoted ECM remodeling through alterations in collagen synthesis and turnover. Transcripts for IGF-binding proteins and Fibromodulin suggest increases in growth and a suppression of myostatin. Overall design: Examination of fully mature myotubes that were treated with either 4% horse serum differentiation media (DMEM, 4%HS, Pen/Strep) or 4% STEM cocktail media (DMEM, 4% STEM, Pen/Strep). Tubes were differentiated in 2% HS differentiation media for 5 days. At day 6 each respective treatment was applied to cells for a duration of 24 hours. After 24 hours cells were collected and RNA isolated and given to the University of Utah Genomics Core for RNA sequencing.