Gene expression in sorted myeloid population from mice

Cells expressing macrophage markers (Cd11b+F4/80+CD169+CX3CR1neg) were sorted from the bone marrow of C57BL/6 mice and their gene expression profile analysed using RNA sequencing. Analysis of the most highly expressed genes revealed a gene expression signature consistent with a granulocyte population (i..e high expression of Ly6g and Cxcr2), rather than macrophages (i.e. low/negligible expression of Adgre1, Cd163 and Mertk). Collectively these data demonstrate that cells which stain positive for conventional macrophage markers exhibit a gene expression signature consistent with cells of the granulocyte lineage. Overall design: RNAseq analysis of sorted Cd11b+F4/80+CD169+CX3CR1neg "macrophages" from the bone marrow of C57BL/6 mice (n=3)