Genome-wide transcripts in NMuMG cells expressing Osr2

Analysis of NMuMG cells expressing Osr2 or treated with TGF-ß. Results provide insight into a novel function of Osr2 in EMT induction. Overall design: Two replicates of total RNA were isolated from NMuMG cells transduced with the Osr2-expressing retroviral vector for 3 days (Day3) or 9 days (Day9) as well as those treated with or without TGF-b (TGFb or Control, respectively) for 2 days. Library construction and RNA-seq were performed by Illumina sequencing platform (Annoroad Gene Technology). Sequence reads were mapped to the mouse genome (GRCm38.p6) using STAR aligner (v2.7.3a) with default parameters. The read counts were calculated by featureCounts (v2.0.0) with the gene model from Ensemble (Mus_musculus GRCm38.75.gtf) at gene level to obtain TPM (Transcripts Per Million). The gene count data was normalized by variance stabilizing transformations (VST) using DESeq2.