RNAseq to understand the host transcriptome during nontuberculous mycobacterial lung infection. Homo sapiens

We sought to elucidate the differentially expressed host factors in bronchoalveolar lavage cells (BALc) from nontuberculous mycobacteria (NTM)-infected subjects by studying the host transcriptome in during infection. A heatmap was created from the normalized expression of selected genes that showed statistically significant differential expression between NTM-infected and uninfected subjects. Genes with similar expression patterns were grouped and the most highly expressed genes are shown. One of the most differentially expressed gene in the NTM-infected group was the CFD (complement factor D) gene. Of note, a statistically significant three-fold increase in expression of the CAMP gene, encoding the LL-37 peptide was observed in the NTM-infected subject group (padj=0.05). Overall design: Bronchoalveolar lavage cell samples from 3 bronchiectatic patients with Mycobacterium intracellulare lung infection and 3 bronchiectatic patients without Mycobacterium intracellulare lung infection