aThe nucleotides incorporated to generate restriction sites are indicated in lower case. bPrimers designed based on R. typhi str. Wilmington genome sequence (NCBI acc. no. NC_006142).
aAll sequences are based on the sequence of the J35 isolate (GenBank accession no. AY386330).bGGATCC is a restriction site of BamHI, AAGCTT is a restriction site of HindIII.
