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Subcellular fractionation and protein digestion based oferential consecutive centrifugationsn dif
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2013-12-31
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Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
1. Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. We found that FASP allows effective processing samples at
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Open Source MALDI Spotter / Fraction Collector
Supplementary data, design files, and code for the manuscript "Open Source Fraction Collector/MALDI Spotter for Proteomics"
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Formaldehyde Derivatization: An Unexpected Side Reaction During Filter-Aided Sample Preparation
The filter-aided sample preparation (FASP) method has been commonly used for proteomic sample preparation due to its high efficiency in removing impurities. Herein, we report an overlooked +12 Da side
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Extending the Compatibility of the SP3 Paramagnetic Bead Processing Approach for Proteomics
The diversity in protein and peptide biochemistry necessitates robust protocols and reagents for efficiently handling and enriching these molecules prior to analysis with mass spectrometry (MS) or oth
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Arg‑C Ultra Simplifies Histone Preparation for LC-MS/MS
Arginine-specific cleavage is the primary method used to prepare lysine-rich histone proteins in bottom-up proteomics. As the Arg-C enzyme has demonstrated suboptimal specificity, cleavage at the carb
Figshare2025-06-12 更新60
