Enhancement of anti-tumor immunity by tertiary lymphoid structures in esophageal squamous cell carcinoma

Our study showed that TLS enhanced anti-tumor immunity in ESCC. We used droplet-based single cell RNA sequencing (scRNA-seq) data of 14 samples, including 10 tumor samples, 4 normal samples. ***14 Samples were re-analyzed from Series GSE197677. The ESCC.integrated.rds processed data includes the re-analyzed 14 Sample data*** data processing step: The sequence data were quantified and mapped to human reference genome (GRCh38) using the Cell Ranger toolkit by 10x Genomics. The matrix data were imported using the Seurat R package (version 3.6.3). To merge the data of all samples, we used the "SCTransform" package for the normalization and variance stabilization of molecular count data.