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Effect of Rom1 silencing on gene expression in Entamoeba histolytica

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE21522
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资源简介:
Entamoeba histolytica trophozoites epigenetically silenced for ameobapore A (G3 parasites) were additionally silenced for the Rhomboid gene Rom1 (EHI_197460) using the mechanism outlined in Mirelman et al. Parasite. 2008 Sep;15(3):266-74 (PMID 18814693). Rom1 silencing was confirmed by RT-PCR. Gene expression in these parasites was compared to that of the parent G3 strain. RNA was isolated from axenically grown G3 and Rom1 silenced parasites, and hybridized to a custom Affymetrix array for E. histolytica. Two biological replicates were performed for each condition.
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2012-03-22
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