Single-cell RNA-seq of mouse peripheral iNKT cells

Invariant natural killer T cells (iNKT) cells are differently distributed in various immune organs. However, it remains unclear whether iNKT cells exhibit phenotypical and functional differences in different peripheral organs and how thymic iNKT migrate to peripheral organs. Hence, characterizing iNKT cells in peripheral organs is important. We profiled the transcriptome patterns of individual mouse peripheral iNKT cells from liver, lymph node, and spleen using a droplet-based single-cell RNA-sequencing (scRNA-seq) approach.