pro-TRH mRNA expression in the brain of zebra finch, Taeniopygia guttata

We show expression of proTRH in the brain of Taeniopygia guttata. The figure shows:

(A) Multiple sequence alignment of prepro-TRH from Xenopus laevis, Danio rerio, Homo sapiens, Rattus norvegicus, and T. guttata showing evolutionary conserved TRH sequences flanked by the basic amino acids. (*) identical residues, (:) residues with strongly similar properties, (.) residues with weakly similar properties. The shaded portions represent TRH progenitor sequences with a great degree of similarity with that of other vertebrate species. (B) qRT-PCR product of pro-TRH mRNA of T. guttata. (C) Relative pro-TRH mRNA levels in the olfactory bulb (OB), left (LT) and right (RT) telencephalon, hypothalamus (Hyp), cerebellum (CB), midbrain (MB), and hindbrain (HB) of T. guttata. The agarose gel shows pro-TRH mRNA expression relative to beta-actin in respective brain regions.