Pregnancy-activated fibroblasts of the mouse mammary gland. Pregnancy-activated fibroblasts of the mouse mammary gland

The mouse mammary gland undergoes severe morphological changes during each pregnancy cycle. These are controlled by epithelial as well as stromal factors, including fibroblasts. This project aimed to identify factors that are expressed in mammary fibroblasts during early pregnancy (day3) when the first morphological changes become microscopically visible. Overall design: Fibroblasts were isolated from three individual 12-13 weeks old mice (2nd, 3rd, and 4th gland) 3 days after plug formation, as well as from three virgin litter-mates as decribed previously (Ibrahim et al., 2017). After RNA isolation and RNA amplification, labelled cDNA was hybridised to Illumina Whole-genome mouse microarrays