Knockout mice lacking MG56

To survey altered gene expression in MG56-knockout muscle, total RNA preparations from mouse hindlimb muscle and hearts were subjected to gene microarray analysis. Results exhibit the effects of MG56 deficiency on overall transcription, and may further imply the biological role of MG56. Total RNA samples were preparated from Tibialis-anterior (TA) muscle and hearts of three P9 MG56-knockout mice and the corresponding wild-type mice were mixed and then subjected to Affymetrix Mus musculus microarrays.为探究MG56基因敲除肌肉组织中的基因表达变化，本研究对小鼠后肢肌肉与心脏的总RNA样本进行了基因芯片分析。该数据集展示了MG56缺失对整体转录水平的调控效应，可为解析MG56的生物学功能提供依据。研究人员从3只出生后第9天（P9）的MG56基因敲除小鼠及其对应野生型小鼠的胫骨前肌（Tibialis-anterior, TA）与心脏中提取总RNA样本，将两类样本分别混合后，采用Affymetrix小家鼠（Mus musculus）基因芯片完成检测分析。