Primer sets and PCR conditions used in this study.
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aIFN-γ primers from ref. [94]; TNF-α primers, ref. [95]; MCP-1 primers, ref. [96]; mCRAMP primers, ref. [13]; iNOS primers, ref. [97]; KC primers, ref. [98]; IL-17A and IL-23p19 ref. [99]; IL-17F primers ref. [36]; and IL-22 primers ref. [100].
bAll PCR experiments had an initial denaturing step of 95°C for 3–5 mins before commencement of PCR cycling conditions.
cMCP1 primers after 40 cycles had an additional 2 steps of 94°C for 30 s, and 50°C for 30 s.
创建时间：
2010-05-13
