ARR1 and DELLA act as transcriptional co-regulators in Arabidopsis.

(A) Heat map representation of the Arabidopsis gene set whose regulation by ARR1ΔDDK:GR or CKs depends on DELLA proteins. The colour scale represents Z-scores. (B) Enrichment of GO categories of all ARR1 target genes in the presence of DELLAs, visualized with ReviGO. (C) Gene expression analysis by RT-qPCR in response to short-term ARR1ΔDDK:GR induction with or without PAC. (D) Gene expression analysis by RT-qPCR in response to short-term induction of gai-1 with or without BA. (E) ChIP analysis of RGA::GFP-RGA at the promoters of six representative common targets for ARR1 and DELLAs. (F) Fold enrichment of GFP-RGA at selected target promoters in the presence (+DEX) vs the absence (-DEX) of ARR1ΔDDK:GR, in F1 seedlings of a cross between RGA::GFP-RGA and 35S::ARR1ΔDDK:GR plants. In this experiment, qPCR values of ChIP samples were normalized per input in each condition (-DEX, and +DEX), and here we show the ratio between those two conditions. (G) ChIP analysis of endogenous RGA at the promoters of six representative common targets for ARR1 and DELLAs, in the wild type and in arr1 arr12 mutants. ChIP was performed with anti-RGA antibodies. For (C-G), data correspond to single biological samples analyzed in triplicates. A second biological sample showed equivalent results.