Estimates of evolutionary pairwise distances A. over the Austrian strains (including the SMB<sub>1</sub> plasma isolate) and their nine closest relatives, B. five major groups (clades), and C. six minor groups (clusters) among clade 2d, all defined according to the clustering in the phylogenetic tree (Fig 1).
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The average numbers of substitutions per site between nucleotide and amino acid sequences are indicated below and above the diagonal, respectively. Estimation of evolutionary distances was conducted in MEGA6 [19] using the MCL and p-distance algorithms for nucleotide and amino acid sequences, respectively. Group 2d-1 contains the Central/Southern European viruses including Austrian strains, and group 2d-5 consists of the Eastern European WNVs. Abbreviations used: At-bd = strain Blood donor/Vienna/2014Austria (KP109691), SMB1 = first passage of the suckling mouse brain isolate from the Austrian blood donor’s plasma, Cz 329 = strain Cz 13–329 (KM203861), Cz 479 = strain Cz 13–479 (KM203862), At-Cx = strain Cx pipiens/Vienna/2014Austria (KP109692), It AN2 = isolate Italy/2011/AN-2 (JN858070), It 32.1 = strain Italy/2013/Rovigo/32.1 (KF588365), It 33.2 = strain Italy/2013/Rovigo/33.2 (KF647249), It 34.1 = strain Italy/2013/Padova/34.1 (KF647251), Cz 104 = s train CZ 13–104 (KM203860), Cz 502 = strain Cz 13–502 (KM203863), At-gh = WNV strain Austria/2008_goshawk (KF179640). Estimates of evolutionary pairwise distances A. over the Austrian strains (including the SMB1 plasma isolate) and their nine closest relatives, B. five major groups (clades), and C. six minor groups (clusters) among clade 2d, all defined according to the clustering in the phylogenetic tree (Fig 1).
