Effects of trehalose on induction of immune responses to unprocessed influenza vaccine injected intramuscular1.

1Virus specific total IgG antibody responses were determined at weeks 1, 2, and 4 after a single immunization of mice (6 BALB/c mice for each group) with inactivated influenza vaccine with or without trehalose.2Vaccines are unprocessed inactivated whole virus (A/PR8i, 0.4 µg), unprocessed inactivated virus plus trehalose (A/PR8i 0.4 µg + 15% trehalose), or mock (15% trehalose).3Virus specific total IgG antibody titers were expressed as the highest dilution having a mean optical density at 450 nm greater than the mean value plus 3 standard deviations of naïve serum samples.