SYBR Green and Lysotracker on Vibrio tappets strains

The aim of this experiment was to mesure the amount of acidic organelles and the number of non-adherent hemocytes after bacterial challenge of hemocytes from the Manila Clam R. philippinarum with FSSW (control), Vibrio tappets CECT4600 (positive control) and Vibrio tappets delta-virB4. Conclusion: The amount of acidic organelles decrease after challenge by V. tappets CECT4600 and delta virB4 as compared to the control, on Manila clam hemocytes The number of non-adherent hemocytes is increased, as compared to the FSSW control, after challenged with the strain V. tappets CECT4600 and V. tappets delta-virB4. Notes: Hemocytes population and amount were me seared using FITC and SSC parameters. On this population, the amount of acidic organelles was measured using APC and SSC