RNAseq of bovine early embryo after miR-17-5p mimic treatment

MicroRNA potentially regulates hundreds of mRNAs but how miRNA impacts on gene expression profile of cells is difficult to examine. Body of literatures have demonstrated presence of miRNAs in oviductal fluid, and these miRNAs are believed to regulate embryo development. Mir-17-5p is one of the major miRNAs in the bovine oviductal fluid. The present data is RNAseq of bovine early embryos after miR-17-5p mimic treatment. Embryos were produced from oocytes of slaughterhouse derived-ovaries and a frozen thawed semen, and the 8-cell stage embryos (2 days after insemination) were removed of their zona pellucida and incubated with miR-17-5p mimic (60nM, Cat 4464066) or control mimic (Cat 4464059) for 2 days, and then subjected to RNA extraction (RNAqueous kit, Life Technologies, Carlsbad, CA, USA). Three batchs of RNA were created using differential ovary series. RNA quality was confirmed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), and cDNA libraries were prepared using the SMARTer kit (Takara, Shiga). Library quality and quantity were determined using the Agilent 2100 Bioanalyzer and the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA), respectively. A multiplexed library was sequenced as 75-bp single reads on a NextSeq500 platform (Illumina, San Diego, CA, USA). Sequence data were filtered to discard the adapter sequence, ambiguous nucleotides and low-quality sequences. The remaining sequence data were aligned to the Bos Taurus genome sequence (ARS-UCD1.2/bosTau9) to count sequence reads.