Whole transcriptome RNAseq analysis of germ cells in Inhba and Inha fetal testis. Whole transcriptome RNAseq analysis of germ cells in Inhba and Inha fetal testis

We performed bulk RNA-Seq on male fetal germ cells isolated from E13.5 and E15.5 testes from activin A-deficient mice (Inhba KO), and from E13.5 testes from mice with greater activin A bioactivity (Inha KO). The aim of this expereiment was to determine how chronic altered activin A bioactivity altered the germline transcriptome. Overall design: Germ cells were isolated from embryonic E13.5 and E15.5 Inhba x Oct4-Gfp WT and KO testes, and E13.5 Inha x Oct4-Gfp WT and KO testes via FACS. Cells were collected from n=3-4 (Inhba) and n=2 (littermate pairs; Inha) animals/age/genotype.