Thermodynamic parameters for the binding of Cu2+ to the apo form of human SOD1 as determined by ITC at 25.0oC.

Thermodynamic parameters, Kb, and n, were determined using a model of two independent sets of binding sites. The standard molar binding free energy () and the standard molar binding entropy () for the binding reaction were calculated using Equations 3 and 4, respectively. The buffer used was 20 mM Tris-HCl buffer (pH 7.4). Errors shown are standard errors of the mean.