Thermodynamic parameters for the binding of Cu2+ to the apo form of human SOD1 as determined by ITC at 25.0oC.
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Thermodynamic parameters, Kb, and n, were determined using a model of two independent sets of binding sites. The standard molar binding free energy () and the standard molar binding entropy () for the binding reaction were calculated using Equations 3 and 4, respectively. The buffer used was 20 mM Tris-HCl buffer (pH 7.4). Errors shown are standard errors of the mean.
创建时间:
2015-12-02



