Inhibition of GBS intracellular survival in cultured BMM.

aBMM were infected in vitro for 2 h with GBS NEM 316 or BM110 at MOI of 10 CFU per macrophage. After three washes in HBSS containing penicillin and streptomycin, infected macrophages were further incubated in RPMI medium containing 10% FCS and antibiotics. At indicated time points, the cells were washed with antibiotic-free HBSS, lysed with saponin, and the CFU estimated by plating serial dilutions of the lysate onto agar plates. ** P<0.0001 compared with RPMI control at the same time point.