Multiome analysis (ATAC + Gene Expression (GEX)) of SUN1 knockout and control CT26 tumors grown in syngeneic mouse model

T cell infiltration is essential for immune checkpoint inhibitors to be effective in treating solid cancers. Through a bioinformatic pipeline, we identified a target gene SUN1 that might relate to modulating immune cell infiltration and immune response. Thus, we generated one Sun1_knockout CT26 cell line (Sun1_KO) using CRISPR-Cas9. By performing multiome single nuclei sequencing using tumors grown in syngeneic model, we set out to understand how mouse Sun1 can affect the regulatory network in tumor cells in vivo. Sun1_KO and Sun1_WT cells were subcutaneously injected in the right flank. Tumors were collected at day 14 post-inoculation, and digested into single cells, which were applied to the preparation of 10x multiome libraries according to the instruction. The resulted GEX library and ATAC library were sequenced separately.