Information of selected genes after evaluation, and characteristics of PCR products and primers used in this analysis.

(a) Accession number of genes found in Fragaria vesca genome (http://www.strawberrygenome.org/andhttp://www.rosaceae.org/) that may be amplified by the designed primer pairs. (b) Theoretical melting temperatures calculated by Oligo Primer Analisys software version 6.65 for each primer. (c) Recommended optimal annealing temperature was calculated by gradient PCR and subsequent PCR efficiency optimization. (d) PCR-product melting temperature as determined by melting curves (e) PCR efficiencies were calculated by LigRegPCR. (f) Known references for genes previously analyzed or described.