Effect of Fumonisin B1 exposure on hepatic gene expression in mice fed a regular diet (CHOW) and in mice fed a High Fat Diet (HFD).

This experiment was designed to investigate the effect of Fumonisin B1 exposure on liver gene expression in male mice fed a regular diet (Chow group) and in obese mice which were fed a High fat diet (HFD). The Fumonisin B1 was provided through the drinking water. Wild-type male mice (C57BL/6J) were randomly divided into four groups. Two groups were fed a chow diet with 10 kcal% fat (CHOW, D12450J, Research Diets) and the other two groups were fed a high-fat diet with 60 kcal% fat (HFD, D12492, Research Diets) for 15 weeks. After 12 weeks of feeding, half of the CHOW and HFD groups were exposed to FB1 (10 mg/kg bw/day) by adjusting every two days the amount of consumed FB1 in the drinking water during 3 weeks in order to maintain a constant level of exposure. Every week, mice were weighed, and water consumption was measured to adjust the quantity of FB1 in the water. Food intake was also monitored. At the end of the experiment, mice were sacrificed to collect plasma and tissue samples. Liver samples were removed, weighed, snap frozen in liquid nitrogen and stored at −80°C prior to gene expression analysis.