Average recoverable concentration, purity and yield of the purified antisense oligonucleotide.

aThe product of round 1 was used as seeds for round 2, and that of round 2 for round 3.
bEach run consisted of 95×100 µl reactions. Target and probe concentration are at 1 nM and 100 nM respectively.
cRound 1 had two duplicate runs. As the first run had been used as seeds, the second run was used for purification and analysis.
dPurity was calculated as peak area % at UV 260 nm.