scRNA-seq of endocrine pancreatic lineages in Isl1CKO mouse model

To understand the pathophysiology of diabetes and to develop more effective treatments, we need to unravel how pancreatic endocrine lineages are generated. ISL1 transcription factor has important role in differentiation, maturation and maintenace of endocrine cells. Eliminating ISL1 in mouse pancreatic endocrine progenitors leads to the diabetic phenotype with altered islet architecture. To evaluate effects of lacking transcription factor on molecular level we performed single cell transcriptomics. Overall design: We created conditional knockout mouse model using Cre-loxP system. Isl1CKO mutants carry genotype Neurod1 Cre/+; Isl1 f/f and mice with genotype Neurod1 Cre/+; Isl1f/+ do not show any phenotype and are used as controls. TdTomato fluorescent protein is used as reporter. 9 days old (P9) pups were sacrificed and pancreases were dissected. 6 samples from each genotype were pooled together. FACSed tdTomato+ cells were loaded to 10x Chromium Next GEM Single cell 3' Reagents kit v.3.1.