Effect of FENDRR on genetic expressions of primary liver cancer based on next generation sequencing

Primary liver cancer (PLC) is the third largest contributor to cancer mortality in the world. PLC is a heterogeneous disease that encompasses several biologically distinct subtypes including hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and combined hepatocellular-cholangiocarcinoma (CHC). The cells were cultured in DMEM medium supplemented with 10% fetal bovine serum and 1% antibiotic-antimitotic solution. The HCC cell line LM3 and ICCA cell line RBE were transfected using Lipofectamine 3000 reagent following the manufacturer's protocol and incubated for 48h for further analysis. The gene expression profiles were analyzed of LM3 and RBE cells transfected with si-FENDRR or si-NC to assess effect of FENDRR on genetic expressions of PLC. Overall design: High-throughput sequencing raw data for 4 samples, A1(LM3) and A2(RBE) are two samples from experimental group (cells with si-FENDRR transfection), C1(LM3) and C2(RBE) are two samples from negative control group (cells with si-NC transfection).