<b>Identifying </b><b><i>de novo</i></b><b> assembly EST-SSR in medicinal plant </b><b><i>Citrullus colocynthis</i></b><b> L.</b>

In this research, the data of the transcriptome sequencing study of the fruit of the <i>C. colocynthis</i> medicinal plant were used in which 83,807 <i>de novo</i> assembly sequences were prepared. The assembled transcripts of the<i> C. colocynthis</i> plant were analyzed to determine the frequency and distribution of microsatellites. From 83807 assembled transcripts using Krait software, 20221 potential microsatellites were found in 15506 transcripts and a total of 3065 primer pairs were identified.