Transcriptional regulation in denervated murine GA muscle

Samples of denervated GA muscle were compared to contralateral controls to determine transcriptional changes associated with skeletal muscle denervation. Denervation was performed as described (Moresi et al., 2010). In brief, adult mice were anesthetized with isoflurane. The sciatic nerve of the left leg was exposed and a 2 mm piece was removed. The right leg remained innervated and was used as a control. Mice were sacrificed and the gastrocnemius muscles was then prepared and snap frozen in liquid nitrogen 14 days after denervation.