Supplementary data for Yang et al., Nature 583:425–430 (2020): Physiologic blood-brain transport is impaired with age by a shift in transcytosis

• 41586_2020_2453_MOESM3_ESM.xlsx — Supplementary Table 1: Single-cell RNA-sequencing meta-data for 745 brain endothelial cells flow-sorted from young (3 m.o.) C57BL/6 mice; columns include cell index, age, replicate, t-SNE coordinates, QC metrics, arterio-capillary-venous zonation call, log-plasma fluorescence index-sort intensity, and log2-CPM expression of Pecam1/Bmx/Nr2f2/Mfsd2a/Cav1. Created June 2020. • 41586_2020_2453_MOESM4_ESM.xlsx — Supplementary Table 2: Spearman rank correlation between expression of each of 19,899 genes and per-cell plasma uptake across the 745 cells in Supplementary Table 1. Created June 2020. • 41586_2020_2453_MOESM5_ESM.xlsx — Supplementary Table 3 (Extended Data Table 4): LC-MS lipidomics of brain microvessels from young (3 m.o., n = 4 pools) and aged (20 m.o., n = 4 pools) mice; absolute concentrations (μM) and within-class mol% for 332 lipid species across 10 lipid classes, with log2 fold change and FDR q-value. Created June 2020. • 41586_2020_2453_MOESM6_ESM.xlsx — Supplementary Table 4: Differential expression results from single-cell RNA-seq of brain endothelial cells from aged (22 m.o.) mice treated with the ALPL small-molecule inhibitor versus vehicle, split by zonation segment (Capillary: 568 DEGs; Vein: 130 DEGs; Artery: 199 DEGs); MAST hurdle test with Bonferroni-adjusted P values. Created June 2020. • README_Yang2020_BBB.docx — This file. Deposit metadata. Created May 2026. • Data_dictionary_Yang2020_BBB.xlsx — Per-variable data dictionary covering every column of the four supplementary tables above. Created May 2026.