Experiment 1 % coverage.xlsx

To determine how DEX treatment in WT and MKO mice leads to muscle atrophy, we measured muscle protein fractional synthesis rates using shot-gun LC-MS/MS analysis after in vivo 2H2O labeling . Mice were treated with PBS or DEX for 10 days. During the final 7 days, mice were also labeled with 2H2O. The sample size for wild type controls treated with PBS (WT-PBS, n=6), wild type treated with DEX (WT-DEX, n=6), MKO treated with PBS (MKO-PBS, n=5) and MKO treated with DEX (MKO-DEX, n=5). The rational for biological replicates of n=5 -6 were used to ensure accurate comparison among each group. Biological replicates were chosen to asses biological variability rather than sole reliance on technical replicates. Controls included WT-PBS treated mice (n=6). Two separate dynamic proteomic experiments were completed and combined for final data analysis. This consisted of experiment 1 that included WT-PBS (n=4) and WT-DEX (n=4). Experiment 2 included the final biological replicates of WT-PBS (n=2), WT-DEX (n=2), MKO-PBS (n=5) and MKO-DEX (n=5). The data sets were combined and annotated as described by the following.
Entry name, accession number, group number, sub group number, number of spectra, number of unique peptides, unique score, percent coverage, coverage map, total intensity, protein molecular weight, database, parent charge, score, sequence, chromatographic peak width, parent mass to charge ratio, delta parent mass and delta parent mass in ppm are shown.