five

Padlock probes and padlock probe primers.

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Figshare2015-12-02 更新2026-04-29 收录
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Note: The 5′- and 3′-ends of the probes that are complementary to the target sequences are underlined. The regions where the two padlock probe-specific primers (RAC1 and RCA2) bind for real-time amplification are in lower case letters. The 5′-end of probe, p-indicates phosphorylation. Ambiguous positions were introduced according to the target sequences (in bold): M = A or C, K = G or T, R = A or G, S = C or G.
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2015-12-02
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