Inguinal white adipose tissue expression profiles of WT mice and Slc35d3 adipose tissue knock-in mice

Slc35d3 adipose-specific knockin (SAKI) mice were generated by crossbreeding mice carrying the Slc35d3 transgene locus controlled by loxp-stop-loxp sequence (LSL-Slc35d3) with adiponectin-derived Cre mice, which is a mouse model that expresses the cre enzyme specifically in adipose tissues. Male 6-week-old Slc35d3 adipose-specific knockin mice (SAKI group) and their control mice (wild-type mice, WT group) were fed a normal standard chow diet (NCD, 10 kal% fat, D09100304, Research Diets, Inc) for 10 weeks. All mice reached the experimental endpoint at 16 weeks of age. Mice in WT group were used as controls. Upon reaching the experimental endpoint, inguinal white adipose tissue (IngWAT) from three SAKI mice and three WT mice were removed for RNA sequencing. Slc35d3 adipose-specific knockin (SAKI) mice were generated by crossbreeding mice carrying the Slc35d3 transgene locus controlled by loxp-stop-loxp sequence (LSL-Slc35d3) with adiponectin-derived Cre mice, which is a mouse model that expresses the cre enzyme specifically in adipose tissues. To investigate the differences in adipose tissue expression profiles between Slc35d3 adipose-specific knock-in (SAKI) mice and wild-type (WT) mice, 6-week-old male mice were fed a normal standard chow diet (NCD, 10 kal% fat, D09100304, Research Diets, Inc) for 10 weeks. WT mice were used as controls. All mice reached the experimental endpoint at 16 weeks of age.