Percentage of functional NTS1.

The total protein content of the NiE, NTFT and NiE fractions was determined by the Amido Black method (Table 3). The amount of total NTS1 in the NiE, NTFT and NiE fractions was determined by ImageJ analysis of Coomassie-stained gels. The number of functional NTS1 in pmoles (Table 3) was converted into a milligram value using an NTS1 molecular mass of 96.5 kDa. The percent functionality was calculated as [(mg NTS1 by ligand binding analysis)/(mg NTS1 by SDS-PAGE analysis)]*100.