SpyCLIP: An easy-to-use and high-throughput compatible platform for efficient characterization of protein-RNA interactions
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https://www.ncbi.nlm.nih.gov/sra/SRP148580
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We have developed a covalent linkage-based SpyCLIP method by taking advantage of SpyTag-SpyCatcher chemistry, which circumvents gel purification steps of captured RBP-RNA needed for all existing CLIP methods, thereby dramatically reducing the technical difficulty and enabling high-throughput application of the CLIP technology. Overall design: SpyCLIP and corresponding input sample for PTBP1, SLBP, RBFOX2 and AGO2 in Lenti-X 293T cell line.
创建时间:
2019-09-23



