CITE-seq - MS Twin Study

We performed single-cell transcriptome, surface proteome and TCR analyses using 10X Chromium Single Cell Immune Profiling Solution (5’ approach) with feature barcoding. We successfully sequenced 8 selected twin pairs discordant for MS (16 individuals in total) + 2 additional healthy MS Twins. This allowed us to pair the high-throughput CyTOF data from 116 Twin samples with a high-resolution map of highly multiplexed surface protein expression and an unbiased in-depth analysis of the transcriptome (single-cell indexing of transcriptomes and epitopes: CITE-seq).

Sequencing raw data and processed gene expression data have been deposited into the GEO repository under the accession numbers GSE193181.

本研究采用10X Chromium单细胞免疫分析解决方案（5'方法）及特征条形码技术，对单细胞转录组、表面蛋白组和TCR进行了分析。成功对8对患有多发性硬化症（MS）的异卵双胞胎（共计16位个体）以及2位额外的健康MS双胞胎进行了测序。这一成果使得我们能够将116个双胞胎样本的高通量CyTOF数据与高度多路复用的表面蛋白表达高分辨率图谱以及转录组（转录组和表位的单细胞索引：CITE-seq）的无偏深入分析进行配对。原始测序数据和基因表达数据分析数据已存入GEO数据库，存取号为GSE193181。